Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK) |
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Authors: | Sannomiya Miriam Rodrigues Clenilson M Coelho Roberta G dos Santos Lourdes C Hiruma-Lima Clélia A Souza Brito Alba R M Vilegas Wagner |
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Institution: | Departamento de Química Organica, Instituto de Química, UNESP, Rua Francisco Degni s/n, Bairro Quitandinha, CP 355, CEP 14800-900 Araraquara, SP, Brazil. miriamsannomiya@pop.com.br |
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Abstract: | The methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3-O-alpha-L-arabinoside, quercetin-3-O-beta-D-galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3-O-alpha-L-arabinoside (95 mg), quercetin-3-O-beta-D-galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by 1H NMR, 13C NMR and UV analyses. |
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Keywords: | Counter-current chromatography Plant materials Flavonoids Amentoflavone |
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