Application of Mixed-Mode Oasis MCX Adsorbent for Chromatographic Separation of Selenomethionine from Antarctic Krill After Enzymatic Digestion |
| |
Authors: | Mariana Siwek Amir Bari Noubar Robert Erdmann Bernd Niemeyer Boris Galunsky |
| |
Affiliation: | (1) Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestrasse 15, 21071 Hamburg, Germany;(2) Institute for Coastal Research, GKSS Research Center, Max-Planck-Strasse 1, 21502 Geesthacht, Germany |
| |
Abstract: | The present communication describes the novel application of mixed-mode adsorbents for bifunctional chromatography. Oasis MCX mixed-mode adsorbent (Waters GmbH, Eschborn, Germany) shows a high binding capacity for Se-Met. The adsorbed selenoamino acid is easily eluted by pH change and addition of organic solvent. Determined adsorption isotherm parameters are suitable for its analytical and preparative chromatographic isolation. A chromatographic column packed with the Oasis MCX adsorbent, designed for a single use solid phase extraction, was successfully applied for bifunctional chromatographic separation of Se-Met from hydrolysed Antarctic krill samples with 85 ± 5% recovery. The MCX-packed column was used for more than 60 runs without any change in its performance. |
| |
Keywords: | Gas chromatography Bifunctional chromatography Oasis MCX adsorbents Selenomethionine |
本文献已被 SpringerLink 等数据库收录! |
|