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Synthesis and chromatographic properties of an HPLC chiral stationary phase based upon human serum albumin |
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| Authors: | E. Domenici C. Bertucci P. Salvadori G. Félix I. Cahagne S. Motellier I. W. Wainer |
| Affiliation: | (1) Centro di Studio del C.N.R. per le Macromolecole Stereordinate ed Otticamente Attive, Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Italy;(2) Université de Bordeaux I (U.A 35 CNRS), 33405 Talence Cedex, France;(3) St. Jude Children's Research Hospital, 38105 Memphis, TN, USA |
| Abstract: | Summary A new HPLC stationary phase was synthesized by thein situ covalent immobilization of human serum albumin (HSA). The protein was immobilized on a commerically available diol column which had been activated with 1,1-carbonyldiimidazole. Initial chromatographic studies show that this phase can be used for chiral separations of enantiomeric solutes and that these separations may reflectin vitro binding to the HSA. The effects of mobile phase composition and temperature on the stereochemical resolutions are reported. |
| Keywords: | Column liquid chromatography Chiral separations Human serum albumin on silica Protein binding |
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