| 高分子量大豆蛋白的溶液制备及组份分离 |
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| 引用本文： | 管娟,田琨,姚晋荣,邵正中,陈新. 高分子量大豆蛋白的溶液制备及组份分离[J]. 高分子学报, 2010, 0(2): 250-254. DOI: 10.3724/SP.J.1105.2010.00250 |
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| 作者姓名： | 管娟 田琨 姚晋荣 邵正中 陈新 |
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| 作者单位： | 教育部聚合物分子工程重点实验室,复旦大学高分子科学系先进材料实验室,上海,200433 |
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| 基金项目： | 国家自然科学基金(基金号20674011);;教育部新世纪优秀人才支持计划(项目号NCET-06-0354);;教育部长江学者和创新团队发展计划(项目号IRT-06-12)资助项目 |
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| 摘 要： | 商品化的大豆分离蛋白(SPI)主要由7S和11S两种球蛋白组成,两者较难分离.同时,由于球蛋白的紧密结构使SPI难以直接溶解于水中.采用盐酸胍和二硫苏糖醇在溶解过程中对SPI进行处理,从而获得了较高浓度,并且蛋白质分子链未发生降解的SPI水溶液.此外,通过采用盐酸胍溶解和透析的方法,获得以7S球蛋白为主的溶液和以11S球蛋白为主的沉淀,比较简单地实现SPI中两种主要组份的初步分离.
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| 关 键 词： | 大豆球蛋白 β-大豆伴球蛋白 透析 二硫键 氢键 |
| 收稿时间： | 2009-07-10 |
PREPARATION OF HIGH MOLECULAR WEIGHT SOY PROTEIN AQUEOUS SOLUTION AND SEPARATION OF ITS MAIN COMPONENTS |
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| GUAN Juan,TIAN Kun,YAO Jinrong,SHAO Zhengzhong,CHEN Xin. PREPARATION OF HIGH MOLECULAR WEIGHT SOY PROTEIN AQUEOUS SOLUTION AND SEPARATION OF ITS MAIN COMPONENTS[J]. Acta Polymerica Sinica, 2010, 0(2): 250-254. DOI: 10.3724/SP.J.1105.2010.00250 |
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| Authors: | GUAN Juan TIAN Kun YAO Jinrong SHAO Zhengzhong CHEN Xin |
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| Affiliation: | The Key Laboratory of Molecular Engineering of Polymers of the Ministry of Education of China;Department of Macromolecular Science;Laboratory of Advanced Materials;Fudan University;Shanghai 200433 |
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| Abstract: | Both solubility tests and reference data proved that commercially available soy protein isolate(SPI) had low solubility in pure water.As an alternative,guanidine hydroxyl chloride(GuHCl) and dithiothreitol(DTT) were used to treat SPI during the dissolution process.After dialysis of GuHCl and DTT,an SPI aqueous solution was obtained.Solubility tests showed this SPI aqueous solution had a relatively high concentration(6 times larger as that directly dissolved in pure water),and sodium dodecyl sulfate-polyacry... |
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| Keywords: | Glycinin Dialysis Disulfide bond Hydrogen bond |
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