Characterization of biomaterials polar interactions in physiological conditions using liquid–liquid contact angle measurements: Relation to fibronectin adsorption |
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Authors: | Elodie Velzenberger Karim El Kirat Gilbert Legeay Marie-Danielle Nagel Isabelle Pezron |
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Institution: | aUniversité de Technologie de Compiègne, UMR CNRS 6600 Biomécanique et Bioingénierie, BP 20529, 60205 Compiègne cedex, France;bCentre de Transfert de Technologie du Mans, 20 rue Thalès de Milet, 72000 Le Mans, France;cUniversité de Technologie de Compiègne, EA Transformations Intégrées de la Matière Renouvelable, BP 20529, 60205 Compiègne cedex, France |
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Abstract: | Wettability of biomaterials surfaces and protein-coated substrates is generally characterized with the sessile drop technique using polar and apolar liquids. This procedure is often performed in air, which does not reflect the physiological conditions. In this study, liquid/liquid contact angle measurements were carried out to be closer to cell culture conditions. This technique allowed us to evaluate the polar contribution to the work of adhesion between an aqueous medium and four selected biomaterials widely used in tissue culture applications: bacteriological grade polystyrene (PS), tissue culture polystyrene (tPS), poly(2-hydroxyethyl methacrylate) film (PolyHEMA), and hydroxypropylmethylcellulose-carboxymethylcellulose bi-layered Petri dish (CEL). The contributions of polar interactions were also estimated on the same biomaterials after fibronectin (Fn) adsorption. The quantity of Fn adsorbed on PS, tPS, PolyHEMA and CEL surfaces was evaluated by using the fluorescein-labeled protein. PolyHEMA and CEL were found to be hydrophilic, tPS was moderately hydrophilic and PS was highly hydrophobic. After Fn adsorption on PS and tPS, a significant increase of the surface polar interaction was observed. On PolyHEMA and CEL, no significant adsorption of Fn was detected and the polar interactions remained unchanged. Finally, an inverse correlation between the polarity of the surfaces and the quantity of adsorbed Fn was established. |
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Keywords: | Liquid/liquid contact angle measurements Biomaterials Fibronectin adsorption Wettability Work of adhesion |
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