Observation of individual transitions in magnetically equivalent spin systems |
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Authors: | Pervushin Konstantin Vögeli Beat |
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Affiliation: | Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, ETH H?nggerberg, CH-8093 Zürich, Switzerland. kopeko@phys.chem.ethz.ch |
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Abstract: | Individual transitions of magnetically equivalent spin systems such as methyl groups residing on isotropically tumbling molecules in solution usually cannot be observed as multiplet-split NMR lines. We propose a pair of NMR experiments, 2D [13C,1Halphaalpha]Methyl and [13C,1Hbetabeta]Methyl HSQC, to overcome this limitation and enable direct and selective observation of individual 1H transitions in 13C-labeled methyl spin systems. Immediate applications include quantitative measurements of 1H-1H residual dipolar couplings (RDC) and cross-correlated relaxation between 1H chemical shift anisotropy and 1H-1H dipole-dipole interactions. The use of the experiments for the measurement of RDCs is demonstrated with two proteins, one weakly aligned by means of Pf1 phages and the other using a naturally present paramagnetic heme group. |
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