Electrophoretic analysis of enzyme-linked immunoglobulins and their clinical significance

The appearance of circulating enzyme-linked immunoglobulin complexes (E-Ig) is common for most enzymes used in clinical biochemical tests. The presence of E-Ig may result in altered enzyme activity in serum and interfere with the measurement of isoenzymes, and is thus of diagnostic importance. E-Ig can be identified by confirming that the binding protein is an immunoglobulin by its reaction with specific anti-human immunoglobulin antibodies. Currently, the presence of E-Ig in an individual is regarded as a benign phenomenon, not indicative of any particular disease process. However, it is becoming clear that E-IgG are closely associated with autoimmune states.