| Research on the Role of Rat TPP- Ⅰ in Neuromedin B Degradation |
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| 作者姓名： | DUPei-ge QIUFang-ping ANLi-ping WANGYan LUGang |
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| 作者单位： | [1]PharmaceuticalCollegeofBeihuaUniversity,Jilin132000,P.R.China [2]CollegeofBiologicalEngineering,ChangchunUniversityofTechnology,Changchun130012,P.R.China |
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| 基金项目： | SupportedbytheScientificResearchFundfortheReturnedOverseasChineseScholars,StateEducationMinistry. |
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| 摘 要： | TPP- Ⅰ (tripeptidyl peptidase- Ⅰ ) protein turnover was studied by observing the role of rat TPP- Ⅰ in neuromedin B(NMB) and other neuropeptides degradations in some physiological situations. The mixtures of rat TPP- Ⅰ with each of NMB. other neuropeptides and Ala-Ala-Phe-MCA were made respectively under the same conditions. The reaction was observed at different timeand monitored by means of high performance liquid chromatography (HPLC) and time-of-flight mass spectrometry (TOF-MS) in vitro. NMB was broken down at the same degree as Ala-Ala-Phe-MCA by rat TPP- Ⅰ and Gly-Asn-Leu was released within 16 h, but other neuropeptides were not digested within 24 h. TPP- Ⅰ is the predominant proteolytic enzyme responsible for the intracellular degradation of neuromedin B. NMB has recently been found to be a good natural substrate for rat lysosomal TPP-Ⅰ.
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| 关 键 词： | TPP-Ⅰ 三肽酶 溶菌酶 蛋白酶 退化现象 CLN2 神经系统 血管紧缩素 疾病 |
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