Modelluntersuchungen zur pepsinkatalysierten Peptidsynthese im wäßrig-organischen Zweiphasensystem |
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| Authors: | Peter Kuhl Andreas Wilsdorf Hans-Dieter Jakubke |
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| Affiliation: | 1. Bereich Biochemie, Sektion Biowissenschaften, Karl-Marx-Universit?t, DDR-7010, Leipzig, Deutsche Demokratische Republik
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| Abstract: | The usefulness of biphasic aqueous-organic solvent systems for pepsin-catalyzed synthesis of model peptidesZ-X-Phe-Phe-OMe (X=Ala, Gln, Leu) has been demonstrated by coupling the correspondingZ-X-Phe-OH with H-Phe-OMe. The influence of various organic solvents on pepsin activity was examined. Some examples are given for the influence of nucleophile and enzyme concentration, bufferpH and organic solvent portion on product yield. Tetrachloromethane and mixtures of ethyl acetate/n-hexane proved to be especially useful allowing syntheses in good yields and within comparatively short reaction times of 2–6 hours. |
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