首页 | 本学科首页   官方微博 | 高级检索  
     


Bimodal Targeting Using Sulfonated,Mannosylated PEI for Combined Gene Delivery and Photodynamic Therapy
Authors:Upendra Chitgupi  Yi Li  Mingfu Chen  Shuai Shao  Marie Beitelshees  Myles Joshua Tan  Sriram Neelamegham  Blaine A. Pfeifer  Charles Jones  Jonathan F. Lovell
Affiliation:1. Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY;2. Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY;3. Abcombi Biosciences Inc., Buffalo, NY
Abstract:Photodynamic therapy (PDT) and gene delivery have both been used to target both cancer cells and tumor‐associated macrophages (TAMs). Given the complex nature of tumor tissue, there could be merit in combining these strategies simultaneously. In this study, we developed a bimodal targeting approach to both cancer cells and macrophages, employing materials conducive to both gene delivery and PDT. Polymers libraries were created that consisted of cationic polyethyleneimine (PEI) conjugated to the photosensitizer pyropheophorbide‐a, with sulfonation (to target selectin‐expressing cells) and mannosylation (to target TAMs). Polyplexes, consisting of these polymers electrostatically bound to DNA, were analyzed for transfection efficacy and cytotoxicity toward epithelial cells and macrophages to assess dual‐targeting. This study provides preliminary proof of principle for using modified PEI for targeted gene delivery and PDT.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号