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Hybrid Minimal Core Streptavidin–Obelin as a Versatile Reporter for Bioluminescence‐based Bioassay |
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| Authors: | Eugenia E Bashmakova Vasilisa V Krasitskaya Alexander N Kudryavtsev Vitaly G Grigorenko Ludmila A Frank |
| Institution: | 1. Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia;2. Siberian Federal University, Krasnoyarsk, Russia;3. Chemistry Department, Lomonosov Moscow State University, Moscow, Russia |
| Abstract: | Ca2+‐regulated photoprotein obelin was genetically fused with a minimum‐sized core streptavidin. Hybrid protein (SAV–OL) was produced by bacterial expression and applied as a specific bioluminescent probe in diverse solid‐phase assays. The obtained results clearly demonstrate specific activity of each domain indicating its proper folding with favorable space orientation. SAV–OL has been shown to be a much more sensitive label than the chemical conjugate of a full‐length streptavidin with obelin. |
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