A luminescence immunoassay test method for determining benzo[a]pyrene in natural water |
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Authors: | N. V. Beloglazova P. S. Shmelin A. S. Novikova I. Yu. Goryacheva |
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Affiliation: | 1.Chernyshevskii Saratov State University,Saratov,Russia;2.Tekhnomash,Moscow,Russia |
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Abstract: | A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is ~0.5 ng/mL. |
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