Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells |
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Authors: | Zhuang Zheng-Fei Liu Han-Ping Guo Zhou-Yi Zhuo Shuang-Mu Yu Bi-Ying and Deng Xiao-Yuan |
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Institution: | MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510631, China; Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China |
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Abstract: | This paper first demonstrates second-harmonic generation
(SHG) in the intact cell nucleus, which acts as an optical indicator
of DNA malignancy in prostate glandular epithelial cells. Within a
scanning region of $2.7~\mu$m$\times 2.7~\mu$m in cell nuclei, SHG
signals produced from benign prostatic hyperplasia (BPH) and
prostate carcinoma (PC) tissues (mouse model C57BL/6) have been
investigated. Statistical analyses ($t$ test) of a total of 405
measurements (204 nuclei from BPH and 201 nuclei from PC) show that
SHG signals from BPH and PC have a distinct difference ($p < 0.05)$,
suggesting a potential optical method of revealing very early malignancy
in prostate glandular epithelial cells based upon induced
biochemical and/or biophysical modifications in DNA. |
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Keywords: | nonlinear microscopy multiharmonic generation diagnostic
applications of nonlinear optics |
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