聚ε-己内酯的微米硅球固定化猪胰脂肪酶促合成

本文采用微米硅球固定化猪胰脂肪酶为催化剂合成聚ε-己内酯, 以期获得具有较高分子量、 良好生物相容性和使用安全性的生物可降解医用高分子材料.

Synthesis of Poly(ε-caprolactone) Catalyzed by Immobilized Porcine Pancreas Lipase on Narrow Distributed Micron Glass Beads

Porcine pancreas lipase(PPL) immobilized on narrow distributed micron glass beads was employed successfully for ring-opening polymerization of ε-caprolactone(CL). Different polymerization conditions such as enzyme concentration, reaction temperature and reaction time were studied. The results showed that Mn of the resulting PCL was significantly increased compared with that catalyzed by PPL. Higher temperature and longer reaction time both contributed to gaining PCL with a higher molecular weight, while the yield had almost no change. In addition, for evaluating the recyclibilty of immobilized PPL on narrow distributed micron glass beads for the ring-opening polymerization of CL, we adopted the most severe reaction conditions(180 ℃, 240 h) in the recycling experiments. It was found that the recovered immobilized PPL could be used again with a compatible high catalytic activity. The highest Mn of 21300 of PCL could be obtained at a mass fraction of 5.18% of the reused immobilized PPL at 180 ℃ for 240 h, which is the highest Mn of PCL catalyzed by PPL. The excellent recyclability of immobilized PPL on narrow distributed micron glass beads is very helpful to its further industry applications.