Development of capillary electrophoresis methods for quantitative determination of taurine in vehicle system and biological media |
| |
Authors: | da Silva Dayse L P Rüttinger Hans H Mrestani Yahia Baum Walter F Neubert Reinhard H H |
| |
Affiliation: | Institute of Pharmaceutical Technology and Biopharmacy, Martin Luther University, Halle-Wittenberg, Germany. |
| |
Abstract: | CE methods have been developed for the determination of taurine in pharmaceutical formulation (microemulsion) and in biological media such as sweat. The CE system with end-column pulsed amperometric detection has been found to be an interesting method in comparison with UV and fluorescence detection for its simplicity and rapidity. A gold-disk electrode of 100 mm diameter was used as the working electrode. The effects of a field decoupler at the end of the capillary, separation voltage, injection and pressure times were investigated. A detection limit of 4 x 10(-5) mol/L was reached using integrated pulsed amperometric detection, a method successfully applied to taurine analysis of the biological samples such as sweat. For taurine analysis of oil-in-water microemulsion, fluorescence detector was the favored method, the detection limit of which was 4 x 10(-11) mol/L. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|