Effects of fatty acids on growth and poly-3-hydroxybutyrate production in bacteria |
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| Authors: | K. W. Lo H. Chua H. Lawford W. H. Lo Peter H. F. Yu |
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| Affiliation: | (1) State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzen, China;(2) Open Laboratory of Chirotechnology of the Institute of Molecular Technology for Drug Discovery & Synthesis and the Dept. of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, China;(3) Department of CSE, Hong Kong Polytechnic University, Hong Kong, China;(4) Dept. of Biochemistry, University of Toronto, Ontario, Canada |
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| Abstract: | The effects of saturated and unsaturated fatty acids (lauric acid, palmitic acid, steric acid, oleic acid, linoleic acid, soybean oil) on Sphaerotilus natans, 0B17 (Pseudomonas sp.), and recombinant Escherichia coli DH5(/pUC19/CAB were studied. Oleic acid enhances Poly-3-hydroxybutyrate (PHB) production in these three bacterial strains, suggesting that the single double bond of the acid activates the polyhydroxylkanoate accumulation enzymatic reaction. Under the effect of lauric acid and linoleic acid, the growth of S. natans and 0B17 were totally inhibited. However, the enhanced PHB accumulation in recombinant E. coli was observed. |
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| Keywords: | Fatty acids poly-3-hydroxybutyrate Sphaerotilus natans recombinant Escherichia coli Pseudomonas sp. |
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