| Microarray of DNA probes on carboxylate functional beads surface |
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| 作者姓名： | 黄承志 李原芳 黄新华 范美坤 |
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| 作者单位： | Institute of Environmental Chemistry, Southwest Normal University, Chongqing 400715, China
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| 基金项目： | 中国科学院资助项目，Subsidizing Program of Chinese University Teachers under the Ministry of Education |
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| 摘 要： | The microarray of DNA probes with 5' -NH2 and 5' -Tex/3' -NH2 modified terminus on 10 um carboxylate functional beads surface in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) is characterized in the preseni paper. it was found that the microarray capacity of DNA probes on the beads surface depends on the pH of the aqueous solution, the concentra-tion of DNA probe and the total surface area of the beads. On optimal conditions, the minimum distance of 20 mer single-stranded DNA probe microarrayed on beads surface is about 14 nm, while that of 20 mer double-stranded DNA probes is about 27 nm. If the probe length increases from 20 mer to 35 mer, its microarray density decreases correspondingly. Mechanism study shows that the binding mode of DNA probes on the beads surface is nearly parallel to the beads surface.
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Microarray of DNA probes on carboxylate functional beads surface |
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| HUANG Chengzhi,LI Yuanfang,HUANG Xinhua,FAN Meikun.Microarray of DNA probes on carboxylate functional beads surface[J].Science in China(Chemistry),2000,43(4). |
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| Authors: | HUANG Chengzhi LI Yuanfang HUANG Xinhua FAN Meikun |
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| Abstract: | The microarray of DNA probes with 5' -NH2 and 5' -Tex/3' -NH2 modified terminus on 10 um carboxylate functional beads surface in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) is characterized in the preseni paper. it was found that the microarray capacity of DNA probes on the beads surface depends on the pH of the aqueous solution, the concentra-tion of DNA probe and the total surface area of the beads. On optimal conditions, the minimum distance of 20 mer single-stranded DNA probe microarrayed on beads surface is about 14 nm, while that of 20 mer double-stranded DNA probes is about 27 nm. If the probe length increases from 20 mer to 35 mer, its microarray density decreases correspondingly. Mechanism study shows that the binding mode of DNA probes on the beads surface is nearly parallel to the beads surface. |
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| Keywords: | DNA probe polystyrene beads microarray hybridization |
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