| Kinetics of the Action of Na2SeO3 on Bacillus subtilis Growth as Studied by Microcalorimetry |
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| 作者姓名： | LIU，Yi ZHAO，Ru－Ming |
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| 作者单位： | [1]DepartmentofChemistry，CollegeofChemistryandMolecularSciences,WuhanUniversity,Wuhan,Hubei430072,China [2]DepartmentofChemistry，CollegeofChemistryandMolecularSciences,WuhanUniversity,Wuhan,Hubei430072,Ch |
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| 基金项目： | theNationalNaturalScienceFoundationofChina (Nos.2 99730 30 30 170 0 10 )，theFoundationofChinese
EducationMinistryforYoungMainstayTeachers |
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| 摘 要： | Microcalorimetric bioassay for acute cellular toxicity is based on metabolic heat production from cultured cells.The biological response to toxicants is the inhibition of the heat production rate in cells,and toxicity is expressed as the concentration of toxicant that is 50% effective in this inhibition(IC50).In this paper,the effect of Na2SeO3 on Bacillus subtilis growth was investigated at 37℃ by microcalorimetry.The relationship between growth rate constants(k) and concentration of Na2SeO3(c) shows a logarithmic normal distribution,and IC50 is 20.3μg/mL.All these thermokinetic information is readily obtained by an LKB 2277-204 heat conduction microcalorimeter.Microcalorimetry is a quantitative,inexpensive,and versatile method for toxicology research.
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| 关 键 词： | 微量量热学 亚硒酸钠 枯草杆菌 动力学 |
Kinetics of the Action of Na_2SeO_3 on Bacillus subtilis Growth as Studied by Microcalorimetry |
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| LIU,Yi LIANG,Hui Gang a CAO,Jin Li a ZHAO,Ru Ming b SHEN,Ping b QU,Song Sheng a YU,Zi Niu c a.Kinetics of the Action of Na2SeO3 on Bacillus subtilis Growth as Studied by Microcalorimetry[J].Chinese Journal of Chemistry,2002,20(2):117-122. |
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| Authors: | LIU Yi LIANG Hui Gang a CAO Jin Li a ZHAO Ru Ming b SHEN Ping b QU Song Sheng a YU Zi Niu c a |
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| Institution: | LIU,Yi LIANG,Hui Gang a CAO,Jin Li a ZHAO,Ru Ming b SHEN,Ping b QU,Song Sheng a YU,Zi Niu c a Department of Chemistry,College of Chemistry and Molecular Sciences,Wuhan University,Wuhan,Hub |
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| Abstract: | Microcalorimetric bioassay for acute cellular toxicity is based on metabolic heat production from cultured cells. The biological response to toxicants is the inhibition of the heat production rate in cells, and toxicity is expressed as the concentration of toxicant that is 50% effective in this inhibition (IC50). In mis paper, the effect of Na2SeO3 on Bacillus subtilis growth was investigated at 37 °C by microcalorimetry. The relationship between growth rate constants (k) and concentration of Na2SeO3 (c) shows a logarithmic normal distribution, and lC50 is 20.3 μg/mL. All these thermokinetic information is readily obtained by an LKB 2277–204 heat conduction microcalorimeter. Microcalorimetry is a quantitative, inexpensive, and versatile method for toxicology research. |
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| Keywords: | microcalorimetry thermochemistry Na 2SeO 3 Bacillus subtilis thermokinetics |
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