| 碱提芥菜多糖alp-2的分离纯化及理化性质分析 |
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| 引用本文： | 李夏兰,魏国栋,王昭晶,蔡婀娜,陈培钦.碱提芥菜多糖alp-2的分离纯化及理化性质分析[J].应用化学,2006,23(11):1304-0. |
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| 作者姓名： | 李夏兰 魏国栋 王昭晶 蔡婀娜 陈培钦 |
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| 作者单位： | 国立华侨大学生物工程系 |
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| 基金项目： | 华侨大学校科研和教改项目;国务院侨务办公室科研项目 |
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| 摘 要： | 经水反复浸提后的包心芥菜残渣经稀碱浸提,浓缩,乙醇沉淀,透析,DEAE-Sepharose-F F色谱分离纯化得到一种白色粉末状多糖ALP-2。经醋酸纤维薄膜电泳和HPLC分析证明,ALP-2为纯品。ALP-2不含蛋白质、核酸,为非淀粉类均一多糖,其相对分子量为459873。ALP-2完全酸水解后HPLC分析确定糖基组成为鼠李糖、阿拉伯糖、葡萄糖、半乳糖和另外一个待确定的组分。
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| 关 键 词： | 包心芥菜 多糖 提取 纯化 理化性质 |
| 文章编号： | 1000-0518（2006）11-1304-03 |
| 收稿时间： | 2005-12-24 |
| 修稿时间： | 2006-03-30 |
Purification and Physicochemical Characteristics of Polysaccharide from Leaf Mustard Isolated with Dilute Alkali |
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| LI Xia-Lan,WEI Guo-Dong,WANG Zhao-Jing,CAI E-Na,CHEN Pei-Qing.Purification and Physicochemical Characteristics of Polysaccharide from Leaf Mustard Isolated with Dilute Alkali[J].Chinese Journal of Applied Chemistry,2006,23(11):1304-0. |
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| Authors: | LI Xia-Lan WEI Guo-Dong WANG Zhao-Jing CAI E-Na CHEN Pei-Qing |
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| Institution: | Department of Bioengineering, Huaqiao University, Quanzhou 362011 |
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| Abstract: | After extraction with hot water for several times,the leaf mustard was dried and then a polysaccharide named ALP-2 was isolated from the dried leaf mustard with dilute alkali extraction,alcohol precipitation,and DEAE-Sepharose FF column chromatography.ALP-2 was identified by means of cellulose acetate film(electrophoresis) and HPLC to be a homogeneous polysaccharide,a non-starch polysaccharide without any(protein) and nucleic acid.Its average molecular weight was 459 750.The analysis by(virtue) of HPLC after its complete hydrolysis by acid showed that ALP-2 mainly consisted of rhamnose,(arabinose),glucose,galactose,and a unknown monosaccharide. |
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| Keywords: | leaf mustard polysaccharide isolation purification physicochemical characteristics |
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