| 分光光度法测定新鲜蔬菜中维生素C的含量 |
| |
| 引用本文： | 李占清,魏海英.分光光度法测定新鲜蔬菜中维生素C的含量[J].中国无机分析化学,2014,4(3):79-81. |
| |
| 作者姓名： | 李占清 魏海英 |
| |
| 作者单位： | ;1.河北大学化学与环境科学学院 |
| |
| 基金项目： | 河北省科技厅科技支撑项目(112764351)资助 |
| |
| 摘 要： | 水溶性的维生素C是一种人体必需的营养物质。维生素C是含有烯二醇基的多羟基内酯结构,利用其水溶液在波长265nm处有较强烈的紫外吸收峰,建立了直接用紫外分光光度法测定新鲜蔬菜中维生素C含量的方法。实验结果表明,维生素C的含量在1.012.0μg/mL内线性关系良好,线性方程为A=0.066 03c+0.019 74(R2=0.997 51),检出限为0.044μg/mL,样品加标回收率为97.5%12.0μg/mL内线性关系良好,线性方程为A=0.066 03c+0.019 74(R2=0.997 51),检出限为0.044μg/mL,样品加标回收率为97.5%105.4%。方法操作简单,准确度高,对新鲜蔬菜的测定结果令人满意。
|
| 关 键 词： | 新鲜蔬菜 维生素C 紫外分光光度法 |
| 收稿时间： | 2014/3/7 0:00:00 |
| 修稿时间： | 2014/3/18 0:00:00 |
Determination of Contents of Vitamin C in Fresh Vegetables by UV Spectrophotometry |
| |
| LI Zhanqing and WEI Haiying.Determination of Contents of Vitamin C in Fresh Vegetables by UV Spectrophotometry[J].Chinese Journal of Inorganic Analytical Chemistry,2014,4(3):79-81. |
| |
| Authors: | LI Zhanqing and WEI Haiying |
| |
| Institution: | College of Chemistry and Environmental Science, Hebei University, Baoding,Hebei 071002,China;College of Chemistry and Environmental Science, Hebei University, Baoding,Hebei 071002,China |
| |
| Abstract: | Soluble vitamin C (Vc) is an essential nutrient for human beings. Vc contains enediol moiety in a polyhydroxy lactone structure A spectrophotometric method for the direct determination of Vc content in fresh vegetables was established, because Vc aqueous solution has relative strong characteristic absorbance at 265 nm. A good linear relationship was obtained in the range of 1.0~12.0 mg/mL of the Vc content. The regression equation was A=0.066 03C+0.019 74 (R2=0.997 51). The detection limit was 0.044 mg/mL with the recoveries of 97.5%~105.4%. The method was simple and accurate. It can meet the requirements for the determination of Vc in fresh vegetable samples with satisfactory results. |
| |
| Keywords: | fresh vegetable vitamin C UV spectrophotometry |
|
| 点击此处可从《中国无机分析化学》浏览原始摘要信息 |
| 点击此处可从《中国无机分析化学》下载免费的PDF全文 |
|
