Detection of bacteria by surface-enhanced Raman spectroscopy |
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| Authors: | Atanu Sengupta Mirna Mujacic E. James Davis |
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| Affiliation: | (1) Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA;(2) Department of Chemical Engineering, University of Washington, Box 351750, Seattle, WA 98195-1750, USA |
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| Abstract: | The detection and identification of dilute bacterial samples by surface-enhanced Raman spectroscopy has been explored by mixing aqueous suspensions of bacteria with a suspension of nanocolloidal silver particles. An estimate of the detection limit of E. coli was obtained by varying the concentration of bacteria. By correcting the Raman spectra for the broad librational OH band of water, reproducible spectra were obtained for E. coli concentrations as low as approximately 103 cfu/mL. To aid in the assignment of Raman bands, spectra for E. coli in D2O are also reported.  Figure Light scattering apparatus used to detect bacteria |
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| Keywords: | Bacteria detection Bacterial pathogens Raman spectroscopy |
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