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介孔材料MCFs的合成及组装青霉素酰化酶的性质研究
引用本文:高波,陈静,朱广山,傅学奇,王春雷,裘式伦. 介孔材料MCFs的合成及组装青霉素酰化酶的性质研究[J]. 高等学校化学学报, 2004, 25(11): 1998-2000
作者姓名:高波  陈静  朱广山  傅学奇  王春雷  裘式伦
作者单位:1. 吉林大学化学学院无机合成与制备化学国家重点实验室, 长春130012;2. 吉林大学生命科学学院, 长春130012
基金项目:国家自然科学基金 (批准号 :2 9873 0 17和 2 0 10 10 0 4),国家“九七三”计划项目 (批准号 :G2 0 0 0 0 775 0 7)资助
摘    要:介孔材料由于具有纳米级规则孔道和巨大的比表面积而在催化、吸附及分离等方面存在较大的应用价值.近年来,由介孔分子筛如MCM-41和SBA-15州等组装功能性材料已成为研究的热点.酶作为高效催化剂有许多优点,但在溶液中易失活,使用后无法回收,有的酶在溶液中还存在自水解问题:将酶组装在介孔材料中制成固定化酶则可解决上述问题.目前已成功地将辣根过氧化物酶

关 键 词:介孔材料MCFs  青霉素酰化酶  活性  稳定性  
文章编号:0251-0790(2004)11-1998-03
收稿时间:2003-12-16

Immobilization and Property of Penicillin G Acylase in the Channel of Silica Mesoporous Material MCFs
GAO Bo ,,CHEN Jing,ZHU Guang-Shan,FU Xue-Qi,WANG Chun-Lei,QIU Shi-Lun. Immobilization and Property of Penicillin G Acylase in the Channel of Silica Mesoporous Material MCFs[J]. Chemical Research In Chinese Universities, 2004, 25(11): 1998-2000
Authors:GAO Bo     CHEN Jing  ZHU Guang-Shan  FU Xue-Qi  WANG Chun-Lei  QIU Shi-Lun
Affiliation:1. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Changchun 130023, China;2. College of Life Science, Jilin University, Changchun 130023, China
Abstract:Silica mesoporous material MCFs with 16.0 nm pore sizes was prepared by using non-ionic block copolymers and the swelling agents, and was used as the support for the immobilization of enzyme. Penicillin G acylase, an enzyme, was assembled in the channel of MCFs by immersion method. The activity and stability of immobilized penicillin G acylase were studied. It was found that the activity and stability of the immobilized penicillin G acylase increased significantly compared to those of free enzyme. The optimum reaction temperature is 60 ℃. After incubation at 60 ℃ for 1 h, the activity of these immobilized penicillin G acylase remains 69%. These results showed that thermostability and durability on heating of the immobilized penicillin G acylase in MCFs was improved remarkably. The silica mesoporous material MCFs with 3-dimensional channel structure is a good support for the immobilization of enzyme.
Keywords:Mesoporous material MCFs  Penicillin G acylase  Activity  Stability
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