Microdetermination of indole-3-acetic acid in vegetable extracts by high-performance liquid chromatography with fluorescence detection |
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| Authors: | R. Budini S. Girotti A.M. Pierpaoli D. Tonelli |
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| Affiliation: | Chemical Institute “G. Ciamician,” University of Bologna, Via Selmi 2, 40126 Bologna, Italy |
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| Abstract: | A HPLC assay method for the quantitation of indole-3-acetic acid in vegetable extracts is described, using fluorescence detection. The method, which is based on extraction in the presence of poly(vinylpyrrolidone), preliminary cleanup of the extract using Sep Pak C18, and separation on a silica column, has been optimized for the determination of IAA in grape berries. The procedure described is determined to be accurate, sensitive, and reliable over time. The detection limit for IAA is about 0.1 ng. |
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| Keywords: | To whom all correspondence should be sent. |
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