Synthesis of 4-azido-2-isopropylamino-6-ethylamino-s-triazine (ring UL14C)

Radioactive 4-azido-2-isopropylamino-6-ethylamino-s-triazine (¹⁴C triazine ring) was synthesized for use as a photoaffinity label to study the site of triazine action in the chloroplast. It was prepared from ¹⁴C 4-chloro-2-isopropylamino-6-ethylamino-s-triazine by forming the trimethylamine salt followed by treatment with sodium azide. ¹⁴C azidotrazine, 6.6 mg, was obtained in a yield of 64% based on ¹⁴C atrazine. The specific activity was 37.4 μCi/mg, and it assayed over 99.5% radiochemically pure after purification by TLC.