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Self-assembly of Micrometer-long DNA Nanoribbons with Four Oligonucleotides |
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| Authors: | Jie Chao Xiangyuan Ouyang Hongzhen Peng Shao Su Lianhui Wang |
| Affiliation: | 1. Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials(IAM),Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM), Nanjing University of Posts & Telecommunications, Nanjing, Jiangsu 210023, China;Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences,Shanghai 201800, China 2. Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences,Shanghai 201800, China 3. Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials(IAM),Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM), Nanjing University of Posts & Telecommunications, Nanjing, Jiangsu 210023, China |
| Abstract: | DNA nanostructures have found widespread applications in areas including nanoelectronics and biomedicine. However, traditional DNA origami needs a long single‐stranded virus DNA and hundreds of short DNA strands, which make this method complicated and money‐consuming. Here, we present a protocol for the assembly of DNA nanoribbons with only four oligonucleotides. DNA nanoribbons with different dimensions were successfully assembled with a 96‐base scafford strand and three short staples. These biotinylated nanoribbons could also be decorated with streptavidins. This approach suggests that there exist great design spaces for the creation of simple nucleic acid nanostructures which could facilitate their application in plasmonic or drug delivery. |
| Keywords: | self-assembly DNA origami nanoribbons |