| 毛细管电泳免疫分析法研究癌胚抗原与抗体相互作用 |
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| 引用本文： | 邓光辉,姜明国,李济权,谭静宇.毛细管电泳免疫分析法研究癌胚抗原与抗体相互作用[J].分析试验室,2006,25(11):54-57. |
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| 作者姓名： | 邓光辉 姜明国 李济权 谭静宇 |
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| 作者单位： | 广西民族大学化学与生态工程学院,南宁,530006;广西民族大学化学与生态工程学院,南宁,530006;广西民族大学化学与生态工程学院,南宁,530006;广西民族大学化学与生态工程学院,南宁,530006 |
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| 摘 要： | 采用毛细管电泳免疫分析法研究癌胚抗原和抗体相互作用.探讨了缓冲体系、癌胚抗原和抗体的配比、进样时间,进样电压等因素对分离检测的影响.结果表明分离电压为14 kV,进样时间为10 s, 在pH值为5.92的Tris-乙酸缓冲体系（TAE）中, 癌胚抗原及其复合物得到较满意的分离.
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| 关 键 词： | 毛细管电泳免疫分析法 癌胚抗原 抗体 |
| 文章编号： | 1000-0720（2006）11-054-04 |
| 收稿时间： | 2006-01-20 |
| 修稿时间： | 2006-01-202006-04-01 |
Study on interaction of carcinoembryonic antigen and antibody by capillary electrophoresis based immunoassay with conductance detector |
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| DENG Guang-hui,JIANG Ming-guo,LI Ji-quan,TAN Jing-yu.Study on interaction of carcinoembryonic antigen and antibody by capillary electrophoresis based immunoassay with conductance detector[J].Chinese Journal of Analysis Laboratory,2006,25(11):54-57. |
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| Authors: | DENG Guang-hui JIANG Ming-guo LI Ji-quan TAN Jing-yu |
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| Institution: | Chemistry and ecological project institute,Guangxi University for Nationalities, Nanning 530006 |
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| Abstract: | Capillary electrophoresis based immunoassay(CEIA) with conductance detector was used to study the interaction of carcinoembryonic antigen and special antibody in the Tris-acetic acid buffer system.The influence factors on the separations including the buffer system,the ratio of antigen to antibody,the injection time and separation voltage were studied.The results showed that carcinoembryonic antigen could be separated with a TAE running buffer of pH 5.92. |
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| Keywords: | Carcinoembryonic antigen Antibody Capillary electrophoresis(CE) Conductance detection |
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