A validated method for analysis of chromium picolinate in nutraceuticals by reversed phase high performance liquid chromatography |
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Authors: | Koll Michael Hoenen Hubertus Aboul-Enein Hassan Y |
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Affiliation: | Pharmaceutical Analysis Laboratory, Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh 11211, Saudi Arabia. |
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Abstract: | A validated high performance liquid chromatographic method was developed for the determination of chromium picolinate in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed-phase Supelcosil LC-18 (250 x 4.6 mm, 5 microm) column. The mobile phase consisted of acetonitrile:water (40:60 v/v) at a fl ow rate of 0.8 mL/min. The UV-detector was set at 264 nm. The developed method showed a good linear relationship in the concentration range from 0.125 to 12.5 microg/mL with a correlation coefficient from 0.999. The limit of detection and limit of quanti fi cation were 0.091 and 0.181 microg/mL, respectively. |
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Keywords: | chromium picolinate dietary supplement nutraceutical analysis high‐performance liquid chromatography |
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