Study of optimum condition for synthesis of [γ-32P]ATP with high specific radioactivity

The synthesis of γ-³²P]ATP with high specific radioactivity was investigated by the enzymatic method. We utilized a part of the glycolytic pathway which consists of three reaction steps. First, we examined the maximum radioactivity of H₃ ³²PO₄ without the decrease of labeling yield. Next, we examined the minimum amount of reagent without the decrease of labeling yield, because some reagents contain the phosphorus species as impurity. In this paper, we propose the optimum condition for synthesis of γ-³²P]ATP with high specific radioactivity. As our calculation, we will be able to obtain about 220 TBq/mmol specific radioactivity using 1.5 GBq of H₃ ³²PO₄.