Biotransformation of Valdecoxib by Plant Cell Cultures |
| |
Authors: | V Molmoori K Srisailam V Ciddi |
| |
Institution: | (1) University College of Pharmaceutical Sciences, Kakatiya University, Warangal, 506 009, Andhra Pradesh, India |
| |
Abstract: | Valdecoxib is a new anti-inflammatory drug that is highly selective for inhibition of the inducible form of cyclooxygenase
(COX-2). In the present study, biotransformation of valdecoxib was investigated in cell cultures of five medicinal plants,
viz., Catharanthus roseus, Azadirachta indica, Capsicum annuum, Ervatamia heyneana, and Nicotiana tabacum. Identification of the biotransformed products was carried out by using high-performance liquid chromatography coupled with
diode array detection and liquid chromatography–tandem mass spectrometry analysis. All the cultures transformed valdecoxib
into more polar compounds, and C. roseus also produced one unknown compound that is less polar than the substrate. The reactions performed by these plant cell cultures
include hydroxylation, methylation, and demethylation. Optimization studies were performed to investigate the effect of the
day of extraction and substrate concentration on biotransformation. |
| |
Keywords: | Valdecoxib Biotransformation Plant cell cultures Catharanthus roseus Azadirachta indica Capsicum annuum Ervatamia heyneana Nicotiana tabacum |
本文献已被 PubMed SpringerLink 等数据库收录! |
|