| HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride |
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| 作者姓名： | Jin Mao YOU Xing Jun FAN Qing Yu OU Qing Cun ZHU |
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| 作者单位： | Jin Mao YOU; Xing Jun FAN; Qing Yu OU; Qing Cun ZHU(Lanzhou Institute of Chemical Physics，Chinese Academy of Sciences 730000)(Department of Chemistry，Qufu Normal University，Qufu，Shandong 273165) |
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| 摘 要： | Atpresentseparationsandquantitativedeterminationsofaminoacidsbymeansofnewfluorescencereagentsforpre-columnorpost-columnderivatizationinRP-HPLCarestillanactivefiled,developmentshavingbeensummarizedbySnyder'.MostaminoacidsdonotshowUVabsorptionin220-254urn,henceinordertoincreasedetectionsensitivityandimproveselectivity,generallyderivatizationreagentsareemployed.Phenylisothiocyanate(PITC)',OPAand3,5-dinitrobenzoylchloride3arewellknownderivatizationreagefltsforthedeterminationofaminocompounds…
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HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride |
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| Jin Mao YOU, Xing Jun FAN, Qing Yu OU, Qing Cun ZHU.HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride[J].Chinese Chemical Letters,1998(3). |
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| Authors: | Jin Mao YOU Xing Jun FAN Qing Yu OU Qing Cun ZHU |
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| Abstract: | A sensitive HPLC method for the detection of amino acids with pre-columnfluorescence derivatization with carbazole-9-yl-acetyl chloride has been developed. This method,in conjunction with a multi-gradient program, offers baseline resolution of the common CRAC-amino acids from a linear acetonitrile gradient. Separation of amino acid derivatives was obtainedon a reversed-phase C18 column. Derivatization and chromatographic conditions were optimized.Amino acid derivatives were eluted in 40 min with good reproducibility. The relative standarddeviations (n=6) at an analytical concentration of 100 pmol are < 4%. The methods described arealso suitable for the analysis of amino acids in different biological samples. |
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| Keywords: | liquid chromatography amino acids fluorescence derivatization carbazole-9-yl-acetylchloride |
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