| HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride |
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| 引用本文： | Jin Mao YOU, Xing Jun FAN, Qing Yu OU, Qing Cun ZHU(Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences 730000)(Department of Chemistry,Qufu Normal University,Qufu,Shandong 273165). HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride[J]. 中国化学快报, 1998, 0(3) |
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| 作者姓名： | Jin Mao YOU Xing Jun FAN Qing Yu OU Qing Cun ZHU(Lanzhou Institute of Chemical Physics Chinese Academy of Sciences 730000)(Department of Chemistry Qufu Normal University Qufu Shandong 273165) |
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| 作者单位： | Jin Mao YOU; Xing Jun FAN; Qing Yu OU; Qing Cun ZHU(Lanzhou Institute of Chemical Physics，Chinese Academy of Sciences 730000)(Department of Chemistry，Qufu Normal University，Qufu，Shandong 273165) |
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| 摘 要： | Atpresentseparationsandquantitativedeterminationsofaminoacidsbymeansofnewfluorescencereagentsforpre-columnorpost-columnderivatizationinRP-HPLCarestillanactivefiled,developmentshavingbeensummarizedbySnyder'.MostaminoacidsdonotshowUVabsorptionin220-254urn,henceinordertoincreasedetectionsensitivityandimproveselectivity,generallyderivatizationreagentsareemployed.Phenylisothiocyanate(PITC)',OPAand3,5-dinitrobenzoylchloride3arewellknownderivatizationreagefltsforthedeterminationofaminocompounds…
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HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride |
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| Jin Mao YOU, Xing Jun FAN, Qing Yu OU, Qing Cun ZHU. HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride[J]. Chinese Chemical Letters, 1998, 0(3) |
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| Authors: | Jin Mao YOU Xing Jun FAN Qing Yu OU Qing Cun ZHU |
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| Abstract: | A sensitive HPLC method for the detection of amino acids with pre-columnfluorescence derivatization with carbazole-9-yl-acetyl chloride has been developed. This method,in conjunction with a multi-gradient program, offers baseline resolution of the common CRAC-amino acids from a linear acetonitrile gradient. Separation of amino acid derivatives was obtainedon a reversed-phase C18 column. Derivatization and chromatographic conditions were optimized.Amino acid derivatives were eluted in 40 min with good reproducibility. The relative standarddeviations (n=6) at an analytical concentration of 100 pmol are < 4%. The methods described arealso suitable for the analysis of amino acids in different biological samples. |
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| Keywords: | liquid chromatography amino acids fluorescence derivatization carbazole-9-yl-acetylchloride |
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