Piezoelectric enzyme sensor with improved temperature characteristics for determining urea in urine |
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| Authors: | Shen Dazhong Kang Qi Wang Xingyong |
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| Institution: | (1) Chemistry College, Shandong University, 250100 Jinan, Peoples Republic of China;(2) Chemical Engineering Department, Jinan Branch of Shandong Institute of Mining and Technology, 250031 Jinan, Peoples Republic of China;(3) China University of Mining and Technology, 221008 Xuzhou, Peoples Republic of China |
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| Abstract: | Based on the conductivity change produced by the urea/urease reaction, a piezoelectric enzyme sensor is applied to the detection of urea in urine. The influence of the variation in solution temperature on frequency measurement is greatly reduced by using a buffer giving solution conditions with a near-zero frequency temperature coefficient for the sensor. The sum of the frequency shifts over the reaction time was used to increase the sensitivity of the determination. The linear range of the method is 1–30  g/ml for urea, with RSDs of 1–2.6%. The detection limit is 0.01 g/ml. |
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| Keywords: | urea urine piezoelectric temperature coefficient enzyme urease sensor |
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