Dielectrophoretic behavior of a single cell when manipulated by optoelectronic tweezers: A study based on COMSOL ALE simulations |
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| Institution: | 1. College of Information Science and Engineering, Northeastern University, Shenyang 110819, China;2. State Key Laboratory of Synthetical Automation for Process Industries, Shenyang 110819, China;1. Research Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Kraków, Poland;2. Bionanopark Ltd., Łódź, Poland;1. CeFEMA, Department of Chemical Engineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, Lisboa 1049-001, Portugal;2. Centro de Química Estrutural, Department of Chemical Engineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, Lisboa 1049-001, Portugal;3. AstraZeneca, Dosage Form Design and Development Department, Gaithersburg, Maryland 20878;1. Department of Electrical and Computer Engineering, Texas A&M University, College Station, USA;2. Department of Biomedical Engineering, Texas A&M University, College Station, USA;1. Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong, China;2. Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong, China;3. T Stone Robotics Institute, The Chinese University of Hong Kong, Shatin, Hong Kong, China;4. Chow Yuk Ho Technology Centre for Innovative Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |
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| Abstract: | Optoelectronic tweezers uses optically induced dielectrophoretic (DEP) force for manipulating cells in aqueous solution, which has shown potential applications in biology and tissue engineering among other possibilities. To effectively design the optoelectronic tweezers (OETs) chip, detailed knowledge about the behavior of cells in response to DEP force in an aqueous layer is needed. In this paper, the trajectories of an SMMC-77721 cell, simulated as a rigid dielectric sphere, in the induced electric field of optical trapping are studied using both an Arbitrary Lagrangian-Eulerian (ALE) method and a particle-tracing method (PTM) available within the COMSOL multiphysics software platform. Because the ALE method involves solving the distorted electric field around the cell and taking a full account of the Maxwell stress tensor (MST), it is expected to provide more accurate predictions about the spherical cell velocity than PTM that involves dipole moment approximation. Our ALE results show noticeably greater cell velocity than that predicted by the classical DEP expression based on dipole moment approximation. The ALE model can help gain new insights for analyzing cell motions in aqueous solution under sophisticated optical spot patterns. |
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| Keywords: | Optoelectronic tweezers Dielectrophoresis ALE Cell fusion |
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