Enantiomeric separation by capillary electrochromatography using monolithic capillaries with sol-gel-glued cyclodextrin-modified silica particles |
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Authors: | Wistuba Dorothee Banspach Leena Schurig Volker |
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Institution: | Institute of Organic Chemistry, University of Tübingen, D-72076 Tübingen, Germany. dorothee.wistuba@uni-tuebingen.de |
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Abstract: | By an on-column sol-gel process, a chiral monolithic stationary phase was prepared by the fusion of permethyl-beta-cyclodextrin-silica (Chira-Dex-silica) particles and by linking them to the internal capillary wall. The resulting monolith is stable toward voltage (30 kV) and pressure (300 bar) and possesses a high efficiency (up to 100,000 theoretical plates per meter). Efficient enantiomeric separation of various chiral compounds by pressure-supported capillary electrochromatography (CEC) was achieved. When comparing this method to capillary liquid chromatography (LC) employing the same column in an unified equipment, CEC shows a twofold higher column efficiency at comparable elution times and hence better resolution factors. |
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Keywords: | Capillary electrochromatography Enantiomeric separation Monolith Permethyl‐β‐cyclodextrin |
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