| 白簕叶总黄酮的提取和纯化及其抑菌试验初探 |
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| 引用本文： | 肖杭,黎云祥,蔡凌云,高侠.白簕叶总黄酮的提取和纯化及其抑菌试验初探[J].光谱实验室,2010,27(6):2130-2134. |
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| 作者姓名： | 肖杭 黎云祥 蔡凌云 高侠 |
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| 作者单位： | [1]西华师范大学生命科学学院．西南野生动植物资源保护教育部重点实验室,四川省南充市师大路1号637002 [2]凯里学院化学系,贵州省凯里市文化北路22弓556003 |
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| 基金项目： | 四川省教育厅重点项目，四川省重点学科项目 |
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| 摘 要： | 观察白簕叶中总黄酮的抑菌作用。采用微波辅助法从白簕叶中提取黄酮化合物,经HPD-600型树脂纯化,通过圆形滤纸片法和二倍稀释法分别对黄酮提取液进行了抑菌试验和最小抑菌浓度(MIC)的测定。HPD-600型树脂纯化后的黄酮纯度达到21.3%,对3种供试菌的抑菌作用为大肠杆菌金色葡萄球菌芽孢杆菌,随着总黄酮浓度的增加,其抑菌作用也增强。白簕叶总黄酮对大肠杆菌、金色葡萄球菌、和芽孢杆菌的MIC分别为原液的1/4、1/2倍和1倍。本方法为白簕的药用价值开发开辟了新途径。
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| 关 键 词： | 白簕 总黄酮 HPD-600大孔树脂 分离纯化 抑菌作用 |
Extraction, Purification, and Antibacterial Effect of Total Flavonoids from Acanthopanan Trifoliatus Leaves |
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| XIAO Hang,LI Yun-Xiang,CAI Ling-Yun,GAO Xia.Extraction, Purification, and Antibacterial Effect of Total Flavonoids from Acanthopanan Trifoliatus Leaves[J].Chinese Journal of Spectroscopy Laboratory,2010,27(6):2130-2134. |
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| Authors: | XIAO Hang LI Yun-Xiang CAI Ling-Yun GAO Xia |
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| Institution: | (College of Life Science. Key Laboratory for Southwest Wildlife Resources Conservation, Ministry of Education, China West Normal University,Nanchong,Sichuan 637002.P.R. China ; Department of Chemistry,Kaili University.Kaili,Guizhou 556003 ,P. R. China) |
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| Abstract: | The total flavonoids from leaves of Acanthopanan trifoliatus were detectded by the filter paper circle method and the doubling dilution. The antibacterial effect showed on the three bacteria： eseherehia coli 〉 staphy lococcus aureu 〉 bacillus subtilis. The minimal inhibitory concentrations （MIC） of the total flavonoids from leaves of Acanthopanan trifoliatus on eseherehia coli,staphy loeoceus aureu and bacillus subtilis are 1/4,1/2 and 1 of the extraction liquid,respectively. This method provides a new approach for the comprehensive utilization of the medicinal value of Acanthopanan tri f oliatus. |
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| Keywords: | Acanthopanan Trifoliatus Total Flavonoids HPD-600 Macroreticular Resin Sparation and Purification Antibacterial Effect |
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