Assessment of electron beam-induced DNA damage in larvae of chestnut weevil,Curculio sikkimensis (Heller) (Coleoptera: Curculionidae) using comet assay |
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| Affiliation: | 1. Yuzuncu Yil University, Science Faculty, Department of Biology, Van 65080, Turkey;2. Boston University, Department of Biology, 5 Cummington St., Boston, MA 02215, USA;3. Missouri University of Science and Technology, Rolla, MO 65409-0010, USA;1. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China;2. The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, West Yuanmingyuan Road, Beijing 100193, China;1. Department of Pathophysiology and Therapeutics of Diabetic Vascular Complications, Kurume University School of Medicine, Kurume, Japan;2. Department of Chemistry, Kurume University School of Medicine, Kurume, Japan |
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| Abstract: | Effect of electron beam treatment on DNA damage in mature larvae of chestnut weevil Curculio sikkimensis (Heller) was assessed using single-cell gel electrophoresis (DNA comet assay). Electrons at acceleration voltages of 0 (control), 300, 750, 1000, and 1500 kV at radiation doses of 1 and 4 kGy were used. Electron beam-treated chestnut larvae showed typical DNA fragmentation, compared with cells from non-treated ones which showed a more intact DNA. Investigations using the comet assay showed that the parameters including tail length, tail moment, olive tail moment as well as the quota of DNA damage at both the doses were significantly larger than the control batch larvae. Thus, this technique could contribute to analytical identification of an effective disinfestation and quarantine treatment. |
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