Carbonate radical anion-induced electron transfer in bovine serum albumin |
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| Institution: | 1. Science Institute, University of Iceland, Sturlugata 7, 101 Reykjavík, Iceland;2. Department of Earth Sciences, University of Hong Kong, Pokfulam Road, Hong Kong;3. GET, CNRS/UMR 5563-Université Paul Sabatier, 14 rue Edouard Belin, 31400 Toulouse, France;1. Área Departamental de Engenharia Química, Instituto Superior de Engenharia de Lisboa, IPL, R. Conselheiro Emídio Navarro, 1959-007 Lisboa, Portugal;2. Centro de Química e Bioquímica, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Ed. C8, Campo Grande, 1749-016, Lisboa, Portugal;3. Departamento de Química e Farmácia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal;1. Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/164-UPA, 1060, Vienna, Austria;2. Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe, S3000ZAA, Argentina;3. Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, Pabellón 2, Buenos Aires, C1428EGA, Argentina;4. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz, 2290 CABA, C1425FQB, Argentina |
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| Abstract: | Reaction of native and thermally denatured bovine serum albumin (BSA) with carbonate radical anion (CO3 ?) has been studied using pulse radiolysis technique. Scavenging of CO3? by native BSA and consequent electron transfer from tyrosine to tryptophan radical has been observed to occur with almost same rate constant (k～1.7×108 dm3 mol?1 s?1) at pH 8.8. Effect of structural changes, due to thermal denaturation, on scavenging of CO3? and the electron transfer process have been studied and discussed in this paper. |
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