双链荧光核酸适体探针用于蛋白质的简便快速检测

发展了一种基于双链荧光核酸适体(F-Aptamer)探针的简单快速检测蛋白质的分析方法.该双链荧光Aptamer探针由一条带荧光标记的Aptamer探针和带猝灭标记的互补DNA组成,当靶蛋白存在时,能形成比双链荧光Aptamer探针更稳定的F-Aptamer/蛋白质复合物,并发出荧光,从而实现对蛋白质的简便快速检测,检测线性范围为6~100 nmol/L,检出限为6 nmol/L.该方法设计简单,对核酸适体分子的大小和空间结构没有要求,可作为一种通用的基于F-Aptamer识别机理的蛋白质检测方法.

Simple Method of Protein Detection Based on Double-stranded Fluorescent Aptamer Probe

A simple method of protein detection was developed using a double-stranded fluorescent aptamer probe. This double-stranded aptamer probe consisted of an aptamer probe labeled with a fluorophore and a short complementary oligonucleotide labeled with a quencher. In the presence of protein, the double-stranded aptamer probe dissociated and F-aptamer/protein complex was formed, leading to fluorescence restoring. Then protein detection was carried out by monitoring fluorescence enhancement, with a liner range of 6—100 nmol/L and detection limit of 6 nmol/L. This design strategy is easy to generalize for any aptamer without prior knowledge of its secondary or tertiary structure, and would be used as a simple and general tool for protein detection.