应用代谢轮廓区分不同致病性副溶血性弧菌

基于tdh, trh和tlh 3个基因区分了不同的致病性副溶血性弧菌. 采用液相色谱(LC)和气相色谱-质谱联用(GC-MS)技术获得不同的致病性副溶血性弧菌的代谢轮廓, 并将其用于区分不同的致病性副溶血性弧菌; 同时以肠杆菌基因间重复共有序列聚合酶链反应技术(ERIC-PCR)及DNA重复序列PCR技术(REP-PCR)为对照, 采用NTsys2.10e软件计算所得结果的相似系数, 并对气相色谱-质谱联用结果进行解析. 结果表明, 根据所得代谢轮廓可以很好地区分不同的致病性副溶血性弧菌; 对气相色谱-质谱联用分析结果解析发现了不同致病性菌株的潜在生物标志物: tdh⁺, trh^-, tlh⁺菌株3种, tdh^-, trh⁺, tlh⁺菌株2种, tdh^-, trh^-, tlh⁺菌株 3种.

Application of Metabolic Profiling for Distinguishing Different Pathogenic Vibrio Parahaemolyticus

Distinguish of different pathogenic vibrio parahaemolyticus are normally based on tdh,trh and tlhgenes.In this research,the metabolic profiles were obtained by liquid chromatography(LC) and gas chromatography/mass spectrometry(GC-MS),and they were used for distinguishing different pathogenic vibrio parahaemolyticus.The results were compared with E.coli gene sequence between the repeat PCR(ERIC-PCR) and DNA repeats-PCR(REP-PCR).The similarity was calculated by NTsys2.10e software.The results showed that different pathogenic Vibrio parahaemolyticus could be distinguished by LC and GC/MS.Potential biomarkers of different pathogenic strains were found by GC-MS.Strain of tdh+,trh-,tlh+ have 3 kinds of potential biomarkers,strain of tdh-,trh+,tlh+ have 2 kinds of potential biomarkers,strain of tdh-,trh-,tlh+ have 3 kinds of potential biomarkers.