Properties of Spreaded Collagen I Monolayers on the Surface of Aqueous tert-Butanol and n-Hexanol Solutions |
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Authors: | A S Fadeev G P Yampol'skaya V M Rudoy S M Levachev V N Izmailova |
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Institution: | (1) Department of Chemistry, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia;(2) Russian Academy of Sciences, Institute of Physical Chemistry, Leninskii pr. 31, Moscow, 119991, Russia |
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Abstract: | Properties of the monolayers of collagen isolated from the sclera of pig's eye are studied at the air–water interface with increasing tert-butanol or n-hexanol concentrations in a subphase. In the case of aqueous n-hexanol solutions, its adsorption on the subphase surface results in the formation of mixed monolayer whose properties depend on n-hexanol concentration in the subphase and the ratio between the number of alcohol and collagen molecules in the monolayer. At higher n-hexanol surface concentration, the phase separation of the monolayer into the domains of the condensed phase of alcohol and fibrous collagen occurs. A decrease in water activity in the presence of tert-butanol leads to a drastic reduction of collagen surface activity. This effect can be explained by both the constrained collagen spreading on the surface of tert-BuOH solutions and adsorption of alcohol molecules on collagen resulting in macromolecule hydrophilization. Alcohol critical concentrations are disclosed above which collagen monolayers are not formed. |
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