Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror |
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| Authors: | Piyawattanametha Wibool Barretto Robert P J Ko Tony H Flusberg Benjamin A Cocker Eric D Ra Hyejun Lee Daesung Solgaard Olav Schnitzer Mark J |
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| Affiliation: | James H. Clark Center for Biomedical Engineering & Sciences, Stanford University, Stanford, California 94305, USA. |
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| Abstract: | Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm x 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of approximately16 degrees . Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz. |
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