| Abstract: | Fast and sensitive method of prednisolone determination at renewable mercury film electrode using differential pulse adsorptive stripping voltammetry (DPAdSV) was successfully developed. The effects of various factors such as: preconcentration potential and time, pulse height, step potential and supporting electrolyte composition were optimized. A linear voltammetric response for analyte was obtained in the concentration range from 0.05 µmol L?1 (18 µg L?1) to 2.25 µmol L?1 (0.81 mg L?1), with a detection limit of 0.01 µmol L?1 for preconcentration time of 20 s. Repeatability of method was determined as RSD % for prednisolone concentration of 0.04 µmol L?1 as 1.6 % (n=9). The proposed method was successfully applied and validated by recovery parameter of prednisolone in simulated sample and pharmaceutical product. |
