| Nafion化学修饰圆盘预富集-石墨炉 原子吸收联用法对牛血超氧化物歧化酶中铜、锌分布状态的研究 |
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| 引用本文： | 徐通敏,周天舒,金利通.Nafion化学修饰圆盘预富集-石墨炉 原子吸收联用法对牛血超氧化物歧化酶中铜、锌分布状态的研究[J].分析化学,1995(2). |
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| 作者姓名： | 徐通敏 周天舒 金利通 |
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| 作者单位： | 华东师范大学化学系 上海 200062 |
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| 摘 要： | 本文研究了Nafion化学修饰钨丝圆盘预富集-石墨炉原子吸收(GFAAS)方法测定牛血超氧化物歧化酶(SOD)中游离态Cu～(2+)及Zn～(2+)的方法,并用GFAAS法直接测定SOD中铜、锌的总量,证实了牛血SOD中金属辅基铜、锌原子个数比为1:1,初步探讨了一定浓度的牛血SOD中Cu～(2+)、Zn～(2+)的表观离解平衡常数。为提高SOD的活性和稳定性的研究,提供了一种有效的方法。
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| 关 键 词： | 化学修饰 石墨炉原子吸收法 超氧化物歧化酶 铜 锌. |
Study on Distribution State of Copper and Zinc in Bovine Superoxide Dismutase by Graphite Furnace Atomic Absorption Spectrometry |
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| Xu Tongmin,Zhou Tianshu,Jin Litong.Study on Distribution State of Copper and Zinc in Bovine Superoxide Dismutase by Graphite Furnace Atomic Absorption Spectrometry[J].Chinese Journal of Analytical Chemistry,1995(2). |
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| Authors: | Xu Tongmin Zhou Tianshu Jin Litong |
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| Abstract: | A method for the determination of free Cu2+ and Zn2+ in bovine superoxide dismu-tase (SOD) solution by GFAAS after preconcentration on Nafion chemically modified tungsten coil is described. The total amount of Cu2+ as well as that of Zn2+ is determined directly by GFAAS. It proves that the atom ratio of Cu and Zn in bovine SOD is 1 : 1. The apparent dissociation constant of Cu2+ and Zn2+ in SOD is also discussed. It provides an effective method to improve the study of SOD' s activity and stabilization. |
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| Keywords: | Nafion chemically modified coil graphite furnace atomic absorption spectrome- try superoxide dismutase copper zinc |
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