The effect of sample salt additives on capillary electrophoresis analysis of intact proteins using surface modified capillaries |
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| Authors: | Anisa Elhamili Magnus Wetterhall Angel Puerta Douglas Westerlund Jonas Bergquist |
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| Institution: | 1. Analytical Chemistry, Department of Physical and Analytical Chemistry, Uppsala University, Biomedical Center, P.O. Box 599, SE-751 24 Uppsala, Sweden;2. Analytical Pharmaceutical Chemistry, Uppsala University, Biomedical Center, P.O. Box 574, SE-751 23 Uppsala, Sweden |
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| Abstract: | The effect of adding alkali salts to protein samples for capillary electrophoretic (CE) analysis of intact proteins was studied. A high degree of peak stacking, even for large proteins, was found to occur when alkali salts were added to the sample. The addition of salt to the protein sample promotes a strong improvement in the peak efficiency of individual proteins giving up to 2.1 × 106 apparent plates/m. The concentration of salt required in the sample to reach optimal peak efficiency show dependency on both the molecular weight and molar concentration of the protein. However, adding salt will, at a sufficiently high concentration, cause a mixture of proteins to co-migrate to one very sharp peak. The observed sample stacking effect was obtained with a number of different surface modified silica capillaries indicating a general phenomenon and not surface coating specific. |
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| Keywords: | Capillary electrophoresis Alkali salt Intact protein analysis Coated capillary Stacking effect |
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