Qualitative and quantitative analysis of aconitine-type and lipo-alkaloids of Aconitum carmichaelii roots |
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| Authors: | Dezs? Csupor Eva Maria Wenzig István Zupkó Karin Wölkart Judit Hohmann Rudolf Bauer |
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| Institution: | 1. Department of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary;2. Institute of Pharmaceutical Sciences, Department of Pharmacognosy, Karl-Franzens University Graz, Universitätsplatz 4, 8010 Graz, Austria;3. Department of Pharmacodynamics and Biopharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary |
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| Abstract: | By optimizing the extraction and analytical conditions, a reliable and precise HPLC method coupled with photodiode array detection (HPLC–DAD) has been developed for the identification and quantification of three major aconitine-type alkaloids (aconitine, mesaconitine, hypaconitine) in the roots of Aconitum carmichaelii Debeaux. The qualitative analysis of the plant material was carried out by LC-APCI-MSn. By means of this method, 26 lipo-alkaloids were also identified from the roots of A. carmichaelii. The effect of processing on aconitine-type alkaloids, lipo-alkaloids and pure aconitine was studied. As part of our investigation, two lipo-alkaloids, 14-benzoylaconine-8-palmitate and 14-benzoylaconine-8-linoleate were produced semisynthetically. The COX-1, COX-2 and LTB4 formation inhibitory activity of aconite root extracts and different types of diterpene alkaloids and the toxicity of lipo-alkaloids were also investigated. |
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| Keywords: | Aconitum carmichaelii Diterpene alkaloids Lipo-alkaloids 14-Benzoylaconine-8-palmitate 14-Benzoylaconine-8-linoleate COX-1 COX-2 LTB4 formation LD50 LC-APCI-MSn |
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