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Simultaneous analysis of anthocyanins and flavonols in petals of lotus (Nelumbo) cultivars by high-performance liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry
Authors:Rui-Zhen Yang  Xiao-Lei Wei  Fen-Fang Gao  Liang-Sheng Wang  Hui-Jin Zhang  Yan-Jun Xu  Chong-Hui Li  Yu-Xuan Ge  Jing-Jing Zhang  Jie Zhang
Institution:1. Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China;2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China;3. Department of Applied Chemistry, China Agricultural University, Beijing 100094, China;4. College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China
Abstract:A fast and reliable HPLC method for the simultaneous separation of anthocyanins and flavonols in lotus petals was developed based on the study of four candidate solvent systems. Fifteen flavonoids were identified by high-performance liquid chromatography with photodiode array detection/mass spectrometry. Among them, two anthocyanins and nine flavonols were discovered in lotus petals for the first time. This work is valuable for both the hybrid breeding on lotus oriented to flower color and the utilization of lotus petals as functional food materials.
Keywords:Lotus  Anthocyanin  Flavonol  HPLC  Mass spectrometry
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