Selective enrichment of aminothiols using polysorbate 20-capped gold nanoparticles followed by capillary electrophoresis with laser-induced fluorescence |
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Authors: | Chien-Chih Shen Wei-Lung Tseng Ming-Mu Hsieh |
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Institution: | 1. Department of Chemistry, National Sun Yat-sen University, 70, Lien-hai Road, Kaohsiung 804, Taiwan;2. Department of Applied Chemistry, Fooyin University, 151, Chinhsueh Road, Ta-liao, Kaohsiung, Taiwan |
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Abstract: | In this article, we report a simple method for selective enrichment of aminothiols using Tween 20-capped gold nanoparticles (AuNPs) prior to capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF). Compared to citrate-capped AuNPs, Tween 20-capped AuNPs exhibit the ability to disperse in a highly saline solution and selectively extract aminothiols through the formation of Au–S bonds. After extraction and centrifugation, 1 mM thioglycollic acid (TGA) was utilized to remove aminothiols that attached to the NP surfaces. After a solution of 8.0 mL aminothiols were extracted using 2× AuNPs (200 μL), the extracted aminothiols derivatized with o-phthalaldehyde at pH 12.0 were detected by CE-LIF. As a result, the limits of detection at a signal-to-noise ratio of 3 for homocysteine (HCys), glutathione (GSH), and γ-glutamycysteine (Glu-cys) are 4013.2, 79.8, and 382.8 pM, respectively. The use of this probe provided approximately 11-, 282-, and 21-fold sensitivity improvements for HCys, GSH, and Glu-cys, respectively. A practical analysis of HCys, GSH, and Glu-cys in human urine sample has been accomplished by this present method. |
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Keywords: | Capillary electrophoresis Gold nanoparticles Enrichment Aminothiol o-phthalaldehyde |
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