Analysis of hydroxylated polycyclic aromatic hydrocarbons in urine using comprehensive two-dimensional gas chromatography with a flame ionization detector |
| |
| Authors: | Leiliane Coelho André Amorim Jean-Marie Dimandja Zenilda de Lourdes Cardeal |
| |
| Institution: | 1. Chemistry Department, ICEx, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil;2. Department of Chemistry, Spelman College, 350 Spelman Lane, SW (Box 279), Atlanta, GA, USA |
| |
| Abstract: | The determination of polycyclic aromatic hydrocarbon (PAH) metabolites in human urine is the method of choice for assessing exposure to carcinogenic compounds. The objective of this study was the development of a comprehensive two-dimensional gas chromatography (GC × GC) method using a flame ionisation detector (FID) to simultaneously determine 10 hydroxylated PAH. The method was based on enzymatic deconjugation, liquid–liquid extraction, and trimethylsilyl (TMS) derivatization of the analytes by microwave heating. Satisfactory separation was achieved. The coefficient of variance was 3.8–12.8%. LOD was 0.03–0.18 μg/L, and LOQ was 0.1–0.5 μg/L. The mean recovery was 76%. The method was applied to the analysis of urine from smokers and non-smokers. |
| |
| Keywords: | GC × GC-FID Hydroxy-PAH Urine analysis |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
