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Methods of calculating protein hydrophobicity and their application in developing correlations to predict hydrophobic interaction chromatography retention |
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| Authors: | Andrea Mahn M Elena Lienqueo J Cristian Salgado |
| Institution: | 1. Department of Chemical Engineering, University of Santiago of Chile, Av. Libertador Bernardo O’Higgins 3363, Santiago, Chile;2. Centre for Biochemical Engineering and Biotechnology, Department of Chemical Engineering and Biotechnology, The Institute for Cell Dynamics and Biotechnology: A Centre for Systems Biology, University of Chile, Santiago, Chile |
| Abstract: | Hydrophobic interaction chromatography (HIC) is a key technique for protein separation and purification. Different methodologies to estimate the hydrophobicity of a protein are reviewed, which have been related to the chromatographic behavior of proteins in HIC. These methodologies consider either knowledge of the three-dimensional structure or the amino acid composition of proteins. Despite some restrictions; they have proven to be useful in predicting protein retention time in HIC. |
| Keywords: | Hydrophobic interaction chromatography Average surface hydrophobicity Hydrophobic contact area Local hydrophobicity Tagged surface hydrophobicity |
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