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The protection effect of β-CD on DNA damage induced by ultrafine TiO2
作者单位:ZHU RongRong(School of Life Science and Technology, Tongji University, Shanghai 200092, China) ; WANG ShiLong(School of Life Science and Technology, Tongji University, Shanghai 200092, China) ; SUN XiaoYu(School of Life Science and Technology, Tongji University, Shanghai 200092, China) ; ZHANG Rui(School of Life Science and Technology, Tongji University, Shanghai 200092, China) ; YAO SiDe(School of Life Science and Technology, Tongji University, Shanghai 200092, China) ;
基金项目:Supported by the National Natural Science Foundation of China (Grant No. 50572074) and Shanghai Special Fund for Nano Materials (05nm05028).
摘    要:Under the photocatalysis of 365 nm ultraviolet radiation, ultrafine TiO2 caused the oxidative damage of Teasy plasmid DNA. The damage was determined by gel-electrophoresis. Then, a different dose of β-CD was added to the reaction, and the damage was restrained. The rate of damage restraining reached 97% when the mass of β-CD was 4 times as that of TiO2. Through UV scan and IR spectroscopy, it was found that the Ti-O of ultrafine TiO2 was bound with -OH of β-CD cavum and the -OH on the surface of ultrafine TiO2 disappeared, so the formation of · OH was controlled. The ultrafine TiO2 has been widely used, but it was determined to be carcinogenic by some research. The protection effect of β-CD to DNA in the molecular level takes a new look on the surface modification of nano particles to decrease the toxic effect.

关 键 词:β-CD    ultrafine  TiO2    plasmid  DNA    oxide  damage    bond  reaction    damage  restrain
收稿时间:10 January 2006
修稿时间:23 June 2006
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