| The protection effect of β-CD on DNA damage induced by ultrafine TiO2 |
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| 作者单位： | ZHU RongRong（School of Life Science and Technology, Tongji University, Shanghai 200092, China） ;
WANG ShiLong（School of Life Science and Technology, Tongji University, Shanghai 200092, China） ;
SUN XiaoYu（School of Life Science and Technology, Tongji University, Shanghai 200092, China） ;
ZHANG Rui（School of Life Science and Technology, Tongji University, Shanghai 200092, China） ;
YAO SiDe（School of Life Science and Technology, Tongji University, Shanghai 200092, China） ; |
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| 基金项目： | Supported by the National Natural Science Foundation of China (Grant No. 50572074) and Shanghai Special Fund for Nano Materials (05nm05028). |
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| 摘 要： | Under the photocatalysis of 365 nm ultraviolet radiation, ultrafine TiO2 caused the oxidative damage of Teasy plasmid DNA. The damage was determined by gel-electrophoresis. Then, a different dose of β-CD was added to the reaction, and the damage was restrained. The rate of damage restraining reached 97% when the mass of β-CD was 4 times as that of TiO2. Through UV scan and IR spectroscopy, it was found that the Ti-O of ultrafine TiO2 was bound with -OH of β-CD cavum and the -OH on the surface of ultrafine TiO2 disappeared, so the formation of · OH was controlled. The ultrafine TiO2 has been widely used, but it was determined to be carcinogenic by some research. The protection effect of β-CD to DNA in the molecular level takes a new look on the surface modification of nano particles to decrease the toxic effect.
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| 关 键 词： | β-CD ultrafine TiO2 plasmid DNA oxide damage bond reaction damage restrain |
| 收稿时间： | 10 January 2006 |
| 修稿时间： | 23 June 2006 |
| 本文献已被 SpringerLink 等数据库收录！ |
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